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Macroscopic Assessment The depth of destruction in articular cartilage was evaluated according to the measures described by Pelletier et al 32 using a four-point scale (0 = normal-appearing surface, 1 = negligible fibrillation or a minor yellowish discoloration of the surface, 2 = destruction spread out into the superficial or central layers only, 3 = destruction that spread into the deep layers, and 4 = destruction that spread to the subchondral bone)

The found enhanced inflammation induced by LZD was associated with a significant reduction of Beclin-1 expression (p 0.05) to about one-third (31.42 2.124 pg/mL) compared to that of the control group (106.7 5.905 pg/mL)

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For data processing, the background fluorescence was subtracted to all fluorimetric signals which were further normalized to mBrB fluorescence recorded in control cells

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